RESUMO
Fresh water fish Labeo rohita was exposed to two pesticides i.e., endosulfan an organchlorine and fenvalerate a synthetic pyrethroid. The 1/10th of 24 hr LC50 of endosulfan (0.0687 μ g l-1) and fenvalerate (0.0 474 μ g l-1) were selected as sub lethal concentrations. The fish were exposed to sublethal concentrations for one week and the changes in the tissue proteins of vital organs such as brain, liver, gill and muscle were studied under SDS-PAGE. The protein subunits were identified by running marker proteins parallel and Rm values were calculated accordingly. The changes in the protein banding pattern are more pronounced in the fenvalerate exposure than endosulfan.
RESUMO
Acute lymphoblastic leukaemia (ALL) is the most common pediatric malignancy worldwide. The origin of this disease may be explained by a combination of genetic and environmental factors. Glutathione-s-transferases are a multi-gene family of enzymes involved in the detoxification of a wide variety of environmental carcinogens. A total of 92 immunophenotyped cases (below 25 years of age) and 150 cord blood controls were here analysed by PCR for GSTM1(Present/Null) and RQ-PCR allelic discrimination assay for GSTP1(Ile105Val). We found a significant increased risk for ALL with the GSTM1 null genotype (OR: 1.96, 95%CI=1.08-3.57), but no significant risk was found with the GSTP1 (Ile/Val) genotype (OR: 1.32, 95%CI = 0.74-2.37) and the GSTP1 Val/Val genotype (OR: 1.41, 95%CI=0.5-3.96) alone. Combined analysis of GSTM1 and GSTP1 showed significant higher risk associated with the GSTM1 (null/null) and GSTP1 [(Ile/Val)/ (Val/Val)] genotype (OR=2.78: 95%CI=1.16-6.69).